Subterranean clover (Trifolium subterraneum) is a key pasture species in Australia that is economically important and a reference for Leguminosae comparative mapping. Despite progress in breeding, further gains are expected if breeders are able to exploit new traits. Recently UWA invested in subterranean clover de novo genome sequencing experiment and generated a draft assembly which provides a platform to proceed to the whole genome sequencing (WGS) (for a high resolution picture of the gene space of subterranean clover). Availability of WGS for subterranean clover will serve as a starting point for developing the first HapMap of the World CORE germplasm collection in subterranean clover. The HapMap will serve as a resource for plant breeders to find genes and design markers readily for any economically important trait with a view to developing climate-smart cultivars.