The Pawsey Supercomputing Centre

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Large complex genome assembly and annotation

In this project two large genomes 1) Barley (10Gb) and 2) South Cattle tick (7Gb) will undergo high throughput analyses. Compared to the human genome  (2.7Gb) they are formidable for Bioinformatics analysis.

1) Barley (Hordeum vulgare L.) is one of the world’s most cultivated crop plants. We will annotate 6 barley genomes assembled on the Pawsey Supercomputing Centre’s resources in 2014, to find key variations in malting quality. Also chromosome 7H 8,000 BAC sequences will be assembled and annotated as part of the Barley International Genome Sequencing Consortium (IBSC).

2) The Southern cattle tick, Rhipicephalus (Boophilus) microplus, is one of the most significant cattle parasites in the world, having populations in most of the world’s tropical/subtropical countries. In 2014 we assembled nearly half the genome (3Gb), to improve this assembly we will incorporate the long PacBio sequence reads.  Once assembled the genome will then be annotated to produce the first reference gene data set for this species.

The scope of our analysis will touch on a range of applications, which include bio-security, biodiversity, ecology, animal and crop diseases and food safety. Many of these organisms under this study have large complex genomes, therefore access to the Pawsey Supercomputing Centre’s Magnus higher memory resources will provide infrastructure to support analysis to improve animal and crop care, biodiversity and environmental protection.

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