The Pawsey Supercomputing Centre

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Cane Toad Genome Sequencing

The aim of the project is to assemble the gnome of the Cane Toad (Bufo marinus). The goal is to apply this knowledge to generate a more targeted approach to biological control, that is specific to the toad, ensuring no native animals will be affected. The project combines some of the latest scientific technology with well-established methods to unravel ~4000 million bases of the Cane toad genome. The first step in our approach has been to undertake shotgun sequencing. This gives us millions of randomly cut short sequences. To date, we have generated over 50x Coverage, ( 50 x 4000 millions bases) which lay the foundation of our genetic map. The second step is to use computational resources to assemble the short sequence. This is why we are applying for the this allocation. The Cane toad genome is 30% more than human genome. It is more complex with multiple repeats.

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